Crystallisation of Pectate Lyase 10A Catalytic Module From Cellvibrio japonicas

dc.contributor.author	Muftah Omar Ali
dc.date.accessioned	2024-12-02T08:40:03Z
dc.date.available	2024-12-02T08:40:03Z
dc.date.issued	2019-12-01
dc.identifier.issn	2518-5454
dc.identifier.uri	http://dspace-su.server.ly:8080/xmlui/handle/123456789/2000
dc.description.abstract	Pectate lyase enzymes degrade pectate and its mechanism is deducible from its structure. pectate lyase 10A (Pel10Acm) from Cellvibrio japonicus NCIMB 10462 was cloned and expressed in E.coli BL21(DE3), purified and characterised, and its crystals produced using a cocktail of precipitants. The Rod cluster crystals of Pel10Acm produced in this study were grown from the precipitating mixture of 0.2M MgCl2, 25% PEG (polyethylene glycol), 2K MME (Monomethyl ether), MgCl2; PEG; MME; 2K, 2000 Daltons).	en_US
dc.language.iso	other	en_US
dc.publisher	جامعة سرت _ Sirte University	en_US
dc.relation.ispartofseries	المجلد التاسع - العدد الثاني - ديسمبر 2019;67-75
dc.subject	Rod cluster crystals	en_US
dc.subject	Pectata lysae enzymes	en_US
dc.subject	IMAC	en_US
dc.subject	Crystallisation Screening	en_US
dc.title	Crystallisation of Pectate Lyase 10A Catalytic Module From Cellvibrio japonicas	en_US
dc.type	Article	en_US