Immunoblot Investigation of Aggrecan Protein in Post-mortem Brain Tissue

Abstract

The MS tissue banks estimated that more than fifty millions patient in the world, each patient cost more than one millions for treatment, has social and economic effects. The breakdown of blood brain barrier considered as autoimmune disease and early events in MS parthenogenesis.

This study include investigation the modification of aggrecan glycoprotein in MS (30 samples) postmortem compared to NAWM (20 samples) via using western blotting. Fifty blocks of postmortem brain tissue were extracted their proteins for protein electrophoresis.

Results indicated that human brain tissue express aggrecan proteins and its fragments were increased in MS active lesion compared to the normal control. The presence of aggrecan fragments considered as evidence of activation of different matrix mattaloproeinase enzymes to help neuron outgrowth axons.